Determination of concetrations of antiepileptic agents in saliva by fluorescence polarization immunoassay technique.
نویسندگان
چکیده
منابع مشابه
Comparison of enzyme-multiplied immunoassay technique with fluorescence polarization immunoassay for determination of gentamicin and tobramycin levels in serum.
We assayed serum gentamicin and tobramycin specimens by the enzyme multiplied immunoassay technique (Syva EMIT) and the fluorescence polarization immunoassay (Abbott TDx). When interassay and intraassay control samples were evaluated, both methods gave an overall coefficient of variation of less than +/- 10%. Using patient serum samples, we obtained excellent correlation with both methods in th...
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We have evaluated a fluorescence polarization immunoassay for thyroxine (T4) using the polarization analyzer IBF-129. Fluorescence polarization could be measured within 20 to 23 seconds after addition of fluorescein labeled thyroxine. The method exhibited excellent specificity and acceptable accuracy. The average intra- and inter-assay coefficients of variation were 4.8 and 12.3%, resepectively...
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Determination of digoxin by fluorescence polarization immunoassay (FPIA) with the Abbott "TDx" is significantly influenced by the concentration of total serum protein. Each 10 g/L increase in serum protein results in an 8% decrease in measured digoxin. Studies with [3H]digoxin confirmed that digoxin binds to the protein pellet during the trichloroacetic acid precipitation step before the immuno...
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A rapid fluorescence polarization immunoassay (FPIA) was optimized and validated for the determination of ochratoxin A (OTA) in rye and rye crispbread. Samples were extracted with a mixture of acetonitrile/water (60:40, v/v) and purified by SPE-aminopropyl column clean-up before performing the FPIA. Overall mean recoveries were 86 and 95% for spiked rye and rye crispbread with relative standard...
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ژورنال
عنوان ژورنال: Rinsho yakuri/Japanese Journal of Clinical Pharmacology and Therapeutics
سال: 1990
ISSN: 1882-8272,0388-1601
DOI: 10.3999/jscpt.21.585